Dear Researcher,

Hepatocyte-like cells derived from human-induced pluripotent stem cells (hiPSC-HLCs) are expected to have important roles in drug screening and regenerative medicine applications. However, hiPSC-HLCs are difficult to produce on a large-scale to obtain relevant numbers required for such applications.

In this webinar, Dr Margarida Serra will describe a study that demonstrates the potential of implementing a novel integrated 3D aggregate culture strategy, using stirred-tank bioreactors (STB), for the scalable production of hiPSC-HLCs.

Webinar outline

• Culture of hiPSCs as three-dimensional (3D) aggregates in stirred-tank bioreactors (STB) operated in perfusion.
• Control of dissolved oxygen concentration and dilution rate along the process and integration of hepatic differentiation after 5 days, in sequential steps for up to 28 days.
• Evaluation of the expression of hepatic markers and albumin production after differentiation of hiPSC 3D aggregates to hiPSC-HLCs.
• Assessment of functional characteristics of hiPSC-HLCs, including glycogen storage and drug metabolization capacity, compared to primary hepatocytes.
• Demonstration for the first time the potential of using dielectric spectroscopy to monitor hiPSC expansion and differentiation in STB.

Cannot attend the live session? Please register anyway, and we will send you a link to watch the On-Demand version.